Composite

Part:BBa_I13410:Design

Designed by: jasonk   Group: iGEM04_MIT   (2005-04-29)


Screening Plasmid Test Construct v2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Has a deletion of the C at base 1194 (at the end of I0500). This is after the transcription start of the Pbad promoter and before the RBS of the transcript, so it should not be significant.


Source

References